Figure 4 shows cerebellar concentrations of anandamide and 2-AG in mice following systemic injection with vehicle, 1 mg/kg URB597, 5 mg/kg NAM, or 16 mg/kg JZL184 (Fig. 4, top panel). Compared to vehicle, URB597 increased anandamide concentration without affecting concentration of 2-AG [F(3,16)=12.04, p<0.05]. In contrast, 16 mg/kg JZL184 increased 2-AG concentration without affecting anandamide concentration [F(3,16)=31.73, P<0.05] whereas 5 mg/kg NAM did not produce a significant increase in 2-AG concentration. The magnitude of increase in 2-AG was 5-fold greater for JZL184 than for NAM. Preliminary analysis also showed that rats treated with JZL184 (16 mg/kg) showed increased whole brain concentration of 2-AG (11.3 nmol/g, n=2), as compared to rats treated with vehicle (5.6 nmol/g, n=2), whereas anandamide concentration was similar in these rats (1.5 and 2.0 pmol/g in vehicle- and JZL184-treated rats, respectively).
