Chunk #38 — MATERIALS — REAGENT SETUP — Urea 8 M, polyacrylamide gel mixture 14% (wt/vol)

Source

Detecting ultralow-frequency mutations by Duplex Sequencing.

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Add 480 g of urea to 466 ml of acrylamide and 100 ml of 10× TBE. Allow the urea to dissolve by slowly stirring with mild heat using a stir plate. After the urea is dissolved, bring the volume up to 1 liter with ddH2O. Store it at 4 °C for up to 1–2 months.