to model a surgical procedure, others have reported a significant impact of isoflurane on the brain phosphoproteome (Kohtala et al. 2016), protein kinases (Tao et al. 2016), and phosphatases (Cao et al. 2014). Given our very brief period of isoflurane exposure prior to tissue collection, the effect of isoflurane on differentially expressed phosphorylated proteins is likely minor; nonetheless, we cannot be confident that isoflurane did not interact with genetic background to alter the phosphorylation status of certain proteins.
