The plates were centrifuged at 5600 g for 2 min at 21°C, and the supernatant was discarded. Samples were washed to remove salt traces and impurities by adding 600 μL of washing buffer and centrifuging plates for 2 min. Washes were performed 3–4 times. Finally, total RNA was eluted in 65 μL of pre-heated RNase-free water (50°C).
