The aqueous phase (upper layer, with approximately 60% of the total volume after the QIAzol was added) was transferred to fresh RNase-free 96-well plates. RNA was precipitated by adding 800 μL of 100% ethanol to the aqueous phase (1.5 volume of the aqueous phase), followed by mixing the samples. The samples were then transferred into the RNeasy 96-well plates to allow the RNA in the solution to bind to the membrane by centrifugation.
