Lymphoblastoid cell lines (LCLs) were created by transformation with Epstein-Barr virus of peripheral blood mononuclear cells isolated from subjects recruited as part of the Collaborative Study on the Genetics of Alcoholism (Begleiter et al., 1995) and interviewed with the SSAGA instrument (Bucholz, et al., 1994). LCLs were from 42 individuals, 21 alcohol dependent (AD) and 21 controls. AD was defined as meeting DSM-IV criteria for alcohol dependence (American Psychiatric Association, 1994). Controls were defined as having consumed at least one drink of alcohol but not meeting any of four definitions of alcohol dependence: DSM-IV (American Psychiatric Association 1994), DSM-IIIR (American Psychiatric Association, 1987), ICD-10 (World Health Organization, 1993), or Feighner definite alcoholism (Feighner et al., 1972); none were dependent on any illicit drug. These individuals were previously studied using a 24 h exposure to ethanol (McClintick et al., 2014).
