2 × 106 LCLs from each of 21 AD and 21 control individuals were seeded in 10 ml of RPMI1640 medium supplemented with 15% FBS, 2 mM glutamine. Cells were cultured in the absence or presence of 75 mM ethanol for 48 h, at which time cells were harvested and lysed with buffer RLT plus, and RNA extractions were conducted using the QIAsymphony RNA extraction protocol.
