Both human adult TC tissue, collected from patients receiving neurological surgeries, and mice cells were disassociated, sorted and sequenced as described elsewhere [31], and deposited in the Gene Expression Omnibus GSE73721 and GSE52564. We also accessed neural progenitor cells (day 17) and mature human neurons (days 57 and 100) from Broad iPSC deposited in the AMP-AD portal [42] and neural progenitor cells and iPSC-derived neurons from [54]. Broad iPSC-derived neurons accessed from the AMP-AD portal were generated using an embryoid body-based protocol to differentiate into forebrain neurons. Wild-type cells used in the protocol were obtained from UConn StemCell Core. RNA was purified using PureLink RNA mini-kit (Life Technologies) and libraries were prepared by Broad Institute’s Genomics Platform using TruSeq protocol. Please refer to Additional file 1: Table S2 for additional information.
