We assembled a cell-type-specific reference panel from publicly available RNA-seq datasets comprising both immunopanning collected or iPSC-derived neurons, astrocytes, oligodendrocytes, and microglial cells from human and murine samples. For immunopanning collected cells, antibodies for cell-type-specific antigens were utilized to bind and immobilize their targeted cell types in order to immunoprecipitate and purify each cell type from the suspensions [30]. cDNA synthesis was accomplished using Ovation RNA-seq system V2 (Nugen 7102) and library prepared with Next Ultra RNA-seq library prep kit from Illumina (NEB E7530) and NEBNext® multiplex oligos from Illumina (NEB E7335 E7500). TruSeq RNA Sample Prep Kit (Illumina) was used to prepare library for paired-end sequence on 100 ng of total RNA extracted from each sample. Illumina HiSeq 2000 Sequencer was used to sequence all libraries [30].
