Chunk #11 — 3. Results — 3.1. Cross-generational THC exposure leads to developmental gene expression abnormalities in the striatum of male and female offspring
To expand on the above work and address the impact of sex, here we characterized relevant gene expression profiles in the ventral and dorsal striatum of F1 male and female offspring (Table 1 and 2). We focused on genes encoding subunits of the CB1 receptor (Cnr1), the NMDA receptor (Grin1, Grin2A, Grin2B), and the AMPA receptor (Gria 1, Gria2). We also included two other important regulators of synaptic function: Dlg4 that encodes for postsynaptic density protein 95 (Psd-95) and Dlgap3 that generates the Sapap3 protein, which interacts with Psd-95. Both genes have been broadly implicated in neuropsychiatric phenotypes in humans and animals (de Bartolomeis et al., 2014; Hall et al., 2015; Rauch and Carlezon, 2013; Wan et al., 2014; Wang et al., 2014; Zuchner et al., 2009). mRNA levels were measured by quantitative reverse transcription PCR at two developmental time points, in adolescent (PND35) and young adult (PND62) F1 animals.
