Chunk #12 — 3. Results — 3.1. Cross-generational THC exposure leads to developmental gene expression abnormalities in the striatum of male and female offspring
The data provided several interesting observations and are shown in Tables 1 and 2. In adolescence, analysis of the combined ventral striatal male and female datasets using two-way ANOVAs revealed interactions between treatment and sex for Cnr1 ((F(3,16)=2.07, p=0.04)), Grin1 ((F(3,16)=4.28, p=0.012)) and Grin2B ((F(3,16)=15.81, p=0.01)), as well as a main effect of sex for Grin2A (p<0.0001), Grin2B (p<0.0001) and Dlg4 (p<0.0001) and Dlgap3 (p<0.0001), indicating sex-specific effects of parental THC exposure on these genes (Table 1). These effects were primarily related to the fact that females tended to have higher expression levels than males (see Female/Male ration in Table 1) that was reduced by THC (Table 3). Some genes show remarkable sex-specific differences in their expression levels (e.g. Dlg4 and Dlgap3). In contrast to the ventral striatum, the pattern of expression in the adolescent dorsal striatum showed no significant treatment and sex interactions, but there was a main effect of drug for Grin2B and sex for Grin2A, Grin2B, Gria1, GriaB, Dlg4 and Dlgap3 (Table 2).
