Chunk #10 — Materials and Methods — DNA extraction, SNP selection and genotyping

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Genetic variation of the growth hormone secretagogue receptor gene is associated with alcohol use disorders identification test scores and smoking.

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Genotyping of the SNPs was made by the LGC Genomics in the UK (http://www.lgcgenomics.com) using the KASPar chemistry—a competitive allele‐specific polymerase chain reaction (PCR) SNP genotyping system performed with fluorescence resonance energy transfer (FRET) quencher cassette oligos. For details on the studied SNPs, see Table 2.