Chunk #9 — Materials and Methods — DNA extraction, SNP selection and genotyping

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Genetic variation of the growth hormone secretagogue receptor gene is associated with alcohol use disorders identification test scores and smoking.

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Two missense SNPs in the GHRL (rs4684677 or Gln90Leu and rs696217 or Leu72Met) and one SNPs in the GHSR (rs2948694) were selected on the basis of previous results (Landgren et al. 2011a, 2010, 2008, 2011c). For closer description of the initial selection of these SNPs, see Landgren et al. (2008). SNPs with a previously reported minor allele frequency of < 5 percent were not included in the present study.