The mean of the forward and reverse values was calculated for each CpG site in each sample. Within each ethnicity, Welch’s two-tailed t tests were used to determine if the mean level of methylation at each CpG dinucleotide differed between sexes. Within controls, Welch’s two-tailed t tests were used to determine if there was a significant difference between African-Americans and Hispanics in overall methylation for all 16 CpG dinucleotides, and, also, using our earlier data to compare African-Americans with Caucasians, and Hispanics with Caucasians (Table 2).
