To get better insights into the specificity of interaction, we mapped the surface of the two proteins according to their conservation score from the ConSurf server (Ashkenazy et al., 2010) (Figure 4C) using 73 LRRs and 20 LPHN-OLFs unique sequences. The analysis indicates that interfacing areas of both proteins are mainly composed of residues with high conservation scores. Interestingly, another highly conserved area proximal to the LPHN3-OLF/FLRT3-LRR interface was observed in both proteins (Figure 4C). In FLRT3-LRR this area matches the Unc5D/FLRT2 complex interface (Seiradake et al., 2014).
