Chunk #41 — Materials and Methods — Virus Production

Source

Sorting nexin 27 regulation of G protein-gated inwardly rectifying K⁺ channels attenuates in vivo cocaine response.

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yes

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pAAV-EF1a-double floxed-EYFP-WPRE-HGH pA (Addgene plasmid 20296) was made into AAV serotype 5 at >1010 GC/ml by Salk Institute Viral Vector Core using standard protocols (referred to as AAV DIO-eYFP). Girk2a-EYFP and Snx27b-ires-GFP were sub-cloned into this vector in at Nhe1 and Asc1 sites and also generated into AAV serotype 5 virus (referred to as AAV DIO Girk2a-eYFP and AAV DIO Snx27b-ires-GFP).