Mice age P24–26 were anesthetized with ketamine/xylazine 10/100mg/kg i.p. and placed in a stereotaxic apparatus. 0.5–1.0µl adeno-associated virus was injected into VTA (x,y,z: +/−0.5mm, −2.4mm, −4.5mm from bregma). Post-operative analgesia was ibuprofen in drinking water for duration of recovery. Animals were allowed to recover at least 12 days prior to use in electrophysiology or behavior experiments. Virus injection was confirmed by presence of GFP/YFP expression in VTA.
