This observation is consistent with the observation that the degree of DE was generally quite small and to detect such small changes requires that the variance for these genes must be relatively low. Connectivity requires sufficient variance to accurately detect gene–gene correlations (see below). Overall, we conclude that DE is not a key selection feature for preference lines derived from genetically diverse HS-CC founders and when viewed in a network context. A similar conclusion was reached on a smaller SH sample (Colville et al., 2017).