For statistical analyses within the groups, each participant's functional brain connectivity map was generated with a threshold at the significance level of the whole-brain cluster, with a corrected alpha level of 0.05. For between-group comparisons, analysis of covariance (ANCOVA) was executed to compare z-value maps between participants with AUD and healthy controls after controlling for covariates such as age, sex, years of education, onset age of alcohol use, and BDI score. The thresholds for voxel-level height and cluster-level extent were set with an FDR-corrected P < 0.05. Seed-level correction was used to apply the FDR separately for each seed ROI by implementing both a voxel-level height threshold and a cluster-level extent threshold.
