2). Mean target coverage among the remaining 6,547 samples was 75X, and 91.4% of target bases were captured at >20X sequencing depth. The subset of rare (allele frequency < 1%) variants in the melanocortin 4 receptor gene (MC4R; Ensemble transcript ID: ENST00000299766) were narrowed to those meeting American College of Medical Genetics and Genomics (ACMG)/Association of Molecular Pathology (AMP) pathogenic or likely pathogenic criteria by an American Board of Genetics and Genomics (AMBGG)-certified clinical laboratory geneticist within the Partners HealthCare Laboratory for Molecular Medicine (Boston, MA, USA) who was blinded to any phenotype information (Richards et al., 2015). Among the 24 rare coding variants analyzed, mean sequencing depth across the 6,547 variants was 80X with a genotype missingness rate of 0%. See also Table S4.
