Michigan Imputation Server (https://imputationserver.sph.umich.edu/index.html#!). selecting the “Mixed population” option. Imputed SNPs with an imputation R2<0.03 and those who failed to meet the missingness, MAF, and HWP thresholds stated above were removed. The first two Multi-Dimensional Scaling (MDS) dimensions were plotted to identify and remove outlier genotypes. The GemTools package in R136,137 and Ward’s hierarchical clustering methods were used to identify individuals of European ancestry.
