All SNPs were genotyped using the TaqMan assay in a 384-well microplate format (Applied Biosystems Inc., Foster City, CA). Briefly, 15 ng of DNA was amplified in a total volume of 7 μl containing an MGB probe and 2.5 μl of TaqMan universal PCR master mix. The amplification conditions were 2 min at 50°C and 10 min at 95°C followed by 40 cycles of 95°C for 25 sec and 60°C for 1 min. Allelic discrimination analysis was performed on the ABI Prism 7900HT Sequence Detection System. To ensure the quality of the genotyping, eight positive and negative control DNA samples were added to each 384-well reaction plate.
