To verify the functions of TAF15 and LINC00665 in glioma cells, the impact on cell proliferation was assessed with the Cell Counting Kit-8 (CCK-8) assay, apoptosis was assessed with flow cytometry, and migration/invasion potential was assessed with transwell assays. As expected, upregulation of TAF15 and LINC00665 expression, respectively, inhibited the proliferation, migration, and invasion of glioma cells and promoted their apoptosis (Figures 1F–1H). Quantitative real-time PCR and microarray analysis showed that LINC00665 expression was upregulated in glioma cells with TAF15 overexpression (Figure 2A; Figure S2B). Furthermore, simultaneous overexpression of TAF15 and LINC00665 resulted in weaker proliferation, migration, and invasion capacity, as well as stronger induction of apoptosis, compared with overexpression of TAF15 or LINC00665 alone (Figures 1F–1H).
