As shown in Figures 1A and 1B, TAF15 expression was downregulated in both glioma tissues and cells. Moreover, high expression of TAF15 was negatively associated with poor prognosis in patients with glioma (Figure S1A). The microarray analysis results showed that the expression level of LINC00665 was decreased in U87 and U251 glioma cells compared to that in human astrocytes (Figure S2A). Similarly, LINC00665 expression was downregulated in glioma tissues compared with that in normal brain tissues (NBTs) and was negatively correlated with the pathological grade of glioma (Figure 1C). An RNA fluorescence in situ hybridization (FISH) assay was used to determine the subcellular location and expression of LINC00665, confirming decreased expression in U87 and U251 glioma cells compared with that in human astrocytes (Figures 1D and 1E).
