Bacteria were grown for 8 h, then seeded directly onto NGM plates with 1 mM IPTG and 50 μg/ml Amp. (Similar results were obtained from bacterial cultures grown for 8-18 h before seeding plates; RNAi results obtained after growth longer than 24 h were sometimes weaker.) Seeded plates were allowed to dry at room temperature and induction was continued at room temperature overnight.
