Chunk #10 — Materials and methods — Liver pathology — Oil red O staining

Source

High Fat Diet-Induced Hepatic 18-Carbon Fatty Acids Accumulation Up-Regulates CYP2A5/CYP2A6 via NF-E2-Related Factor 2.

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Oil red O staining was performed according to the method described in our previous study (Wang X. et al., 2016). The fat accumulated in hepatocytes was demonstrated intuitively as red.