Chunk #11 — Materials and methods — Cell culture and treatments

Source

High Fat Diet-Induced Hepatic 18-Carbon Fatty Acids Accumulation Up-Regulates CYP2A5/CYP2A6 via NF-E2-Related Factor 2.

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HepG2 cells used in our experiments were obtained from Harbin Medical University, China. The passage number of cells used in each experiment is 3 or 4. All assays were performed with nine replicates.