Chunk #15 — Methods — RNA isolation for gene expression studies

Source

Transcriptomic Analysis of Induced Pluripotent Stem Cells Derived from Patients with Bipolar Disorder from an Old Order Amish Pedigree.

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Undifferentiated iPSCs, iPSC-derived NPs or neurons at E and L stage of differentiation were rinsed with 1X DPBS followed by RNA extraction using RNeasy Mini kit (Qiagen) using the manufacturer’s protocol. RNA was quantitated using a Nanodrop Spectrometer ND-1000 (Thermo Scientific).