To control ethanol dosage in culture, we utilized an intermittent ethanol exposure (IEE) paradigm as in previous studies [25]. Ethanol was replenished daily to account for loss by evaporation, producing mean concentrations of 15.4 ± 1.16 mM (Supplementary Fig. 7). The half-life of ethanol is 14.5 h (with a 95% CI of 13.5 to 15.6 h), so over 24 h the concentration would drop to ~5–8 mM before replenishment. We targeted a peak concentration of 20 mM ethanol, which is similar to a blood alcohol concentration (BAC) of 0.08% (17 mM), the legal limit for intoxication. We also selected this concentration based on concentration-dependent interactions of GIRK2 with ethanol [17]. After 7 days of IEE, we evaluated neuronal morphological and functional properties. Focusing on parameters that were different by genotype without IEE (Figs. 3, 4), we found that ethanol eliminated the differences in total neurite area (p = 0.98, Fig. 5A.d), GIRK2 puncta counts (p = 0.46, Fig. 5B.a), excitation following current injection (p = 0.32, Fig. 5E.a), step- (p = 0.48, Fig. 5E.b), and ramp- (p = 0.95, Fig.
