(Assay ID: C_15868122_10) were used. Real-time PCR was performed in a final volume of 10 µl containing 2×LightCycler® 480 Probes Master (Roche Diagnostics K.K.), 40×TaqMan® Gene Expression Assays, 5 ng genomic DNA as the template, and up to 10 µl H2O equipped with 2×LightCycler® 480 Probes Master. The thermal condition was the following: 95°C for 10 min, followed by 45 cycles of 95°C for 10 s and 60°C for 60 s, with a final cooling at 50°C for 30 s. Afterward, endpoint fluorescence was measured for each sample well, and the A/A, A/G, and G/G genotypes were determined based on the presence or absence of each type of fluorescence.
