Chunk #18 — Methods — Genotyping

Source

Association between KCNJ6 (GIRK2) gene polymorphisms and postoperative analgesic requirements after major abdominal surgery.

Embedded

yes

Text

For samples that were difficult to genotype for KCNJ6 G-1250A and KCNJ6 A1032G using the PCR-RFLP method, direct sequencing was adopted to determine the sequence with both forward and reverse primers enclosing the SNP sites.