can mimic one group of neurons receiving 2–4 different types of inputs. Different subtypes of human iNs survive in co-culture conditions within the device through 4 weeks and are primarily maintained within their respective compartments, while communicating to the central chamber via microchannels. The neurons display the morphology of mature neurons by expressing both pan-neuronal markers and subtype-specific markers, and demonstrate single-cell functionality and functional connectivity between chambers. Furthermore, near the walls separating side chambers, axonal projections extended through the microchannels toward the same population of central chamber neurons, suggesting the formation of a three-way circuit. This is significant for circuit modeling as many brain nuclei receive inputs from different regions of the brain.
