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Chunk #42 — STAR METHODS — METHOD DETAILS — Culture of various principal types of cells

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ApoE2, ApoE3, and ApoE4 Differentially Stimulate APP Transcription and Aβ Secretion.
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Human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. H1 human ES cells were obtained from WiCell Research Resources (Wicell, WI), maintained in feeder-free condition using mTeSR1 medium (Stem Cell Technologies), and used at intermediate (~50) passage numbers to generate human induced neuronal (iN) cells (Zhang et al., 2013). Cells were cultured as described (Zhang et al., 2013).