Human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. H1 human ES cells were obtained from WiCell Research Resources (Wicell, WI), maintained in feeder-free condition using mTeSR1 medium (Stem Cell Technologies), and used at intermediate (~50) passage numbers to generate human induced neuronal (iN) cells (Zhang et al., 2013). Cells were cultured as described (Zhang et al., 2013).