CD1 wildtype mice Crl:CD1(ICR) purchased from Charles River Laboratories were used in vitro for dissociated cultures of hippocampal neurons, glia and murine embryonic fibroblasts (MEFs), and in vivo for AAV injections (see METHOD DETAILS). Both male and female animals were used, and randomly allocated to experimental groups. All animal usage followed the guidelines of and was overseen by the Administrative Panel on Laboratory Animal Care (APLAC) at Stanford University, the Public Health Service Policy on Humane Care and Use of Laboratory Animals, and the Panel on Euthanasia of the American Veterinary Medical Association. All animals were housed in the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC) accredited Research Animal Facility at Stanford Institutes of Medicine.
