Serum levels of TG were measured using commercially available kits (Pointe Scientific, Canton, MI, USA). Serum FGF21 was measured using a mouse FGF21 kit (BioVendor, Asheville, NC, USA). Liver homogenates were prepared in ice-cold 0.15 M potassium chloride (KCL) and liver TG content was measured as the same manner as serum TG.