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Chunk #10 — 1. MATERIALS AND METHODS — 1.3 Quantification of triglyceride (TG) and FGF21

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Alcoholic fatty liver is enhanced in CYP2A5 knockout mice: The role of the PPARα-FGF21 axis.
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Serum levels of TG were measured using commercially available kits (Pointe Scientific, Canton, MI, USA). Serum FGF21 was measured using a mouse FGF21 kit (BioVendor, Asheville, NC, USA). Liver homogenates were prepared in ice-cold 0.15 M potassium chloride (KCL) and liver TG content was measured as the same manner as serum TG.