Liver sections were stained with hematoxylin and eosin (H&E) for pathological evaluation as described before (Lu et al., 2010; Hong et al., 2015). Steatosis was quantified as the percentage of cells containing lipid droplets. Five fields (200×) per liver section were examined (one 200× field area contains about 200 cells). Steatosis scoring was based on the following criteria: 0, none; 1, <5%; 2, 5–33%; 3, 34–66%; 4, >67% of cells displaying fat accumulation.
