100 bp templates were sequenced using the Ion Sequencing Kit and user guide (Publication 4469714 Rev. C). Manually prepared 200 bp templates were sequenced using the Ion Sequencing 200 Kit, using methods outlined in the corresponding User Guide (Publication 4471999 Rev. B), while 200 bp templates prepared using the OneTouch system were sequenced using the Ion PGM 200 Sequencing Kit and the associated User Guide (Publication 4474246 Rev. B). The Ion Torrent Suite 2.0.1 was used for all analyses and the SFF was subsequently downloaded for analysis. Test runs for D. maricopensis either failed to produce viable libraries, or produced very few reads of low quality, so sequencing this species was no longer pursued.
