Using the Agilent 2100 Bioanalyzer (Agilent Technologies) with the High Sensitivity DNA Kit (Agilent Technologies), the quality, size and concentration of the libraries was determined and the library was diluted, prior to template preparation, so as to keep polyclonal values in the sequencing results to a minimum. For 100 bp libraries, the Ion OneTouch system (composed of the Ion OneTouch Instrument and Ion OneTouch ES) was used to prepare the template, using the methods outlined in the Ion OneTouch Template Kit and associated User Guide (Publication 4468007 Rev. E). 200 bp libraries were prepared either manually, using the Ion Xpress Template 200 Kit and associated User Guide (Publication 4471974 Rev. C), or using the Ion OneTouch system, using the Ion OneTouch 200 Template Kit and associated User Guide (Publication 4472430 Rev C.).
