We used MAGMA (22) to perform a gene-based association analysis; which identified 40 genes that were significantly associated with AUDIT total score (p < 2.70 × 10−6; Supplementary Table 15, Supplementary Figure 15). As expected, the majority of these genes were in the 10 GWAS loci (i.e. KLB, GCKR); DRD2 and CRHR1 were also among the top hits. In addition, the analysis revealed a strong burden signal in CADM2 (p = 1.64 × 10−9), where the index variant in GWAS meta-analysis did not reach genome-wide significance. We did not identify any canonical pathways that were significantly associated with AUDIT (Supplementary Table 16).
