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Chunk #37 — Materials and Methods — Functional Magnetic Resonance Imaging — Data analysis

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The neural correlates of the unified percept of alcohol-related craving: a fMRI and EEG study.
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The fMRI data were analyzed using Statistical Parametric Mapping 8 (SPM8) (The Wellcome Trust Center for Neuroimaging, London, UK) (http://www.fil.ion.ucl.ac.uk/spm). The structural T1 image (retaining the gray matter, white matter, and CSF) was first skull-stripped. The functional images were realigned to the first scan to correct for head movement. The high-resolution skull-stripped T1 image was then co-registered to the mean realigned functional image and subsequently normalized to the Montreal Neurological Institute (MNI) coordinates. Finally, spatial smoothing was applied using a 6 × 6 × 6 mm full-width at half maximum (FWHM) of the Gaussian smoothing kernel. The functional activation was analyzed using a standard general linear model (GLM). A boxcar reference function was convolved with the hemodynamic function to represent the time course of the expected signal during the experiment.