Full-length KIAA1212 was cloned from a mouse cDNA library and inserted into pCRII zero (Invitrogen) or pRK5 vector (BD Pharmingen) for the HA tag. Full-length KIAA1212 and CA-AKT (constructed by replacing its N-terminal pleckstrin homology domain with a myristoylation signal from the Src, Upstate) (Kohn et al., 1996) were subcloned into retroviral expression vector pCUXIE. Fragments of KIAA1212-NT (amino acids 1-240), M1 (amino acids 241-1000), M2 (amino acids 1001-1383), C1 (amino acids 1384-1600), C2 (amino acids 1601-1845) were cloned into the GST-fusion expression vector (pGEX-4T-1; Amersham-Pharmacia Biotech) to fuse GST to the N-terminus of KIAA1212 fragments. Full length KIAA1212 (amino acids 1-1845), M and C (amino acids 241-1845), M2 and C (amino acids 1001-1845), CT (amino acids 1384-1845), CT2 (amino acids 1601-1845) were cloned into pCMVtag1 for the FLAG tag. Full-length mouse DISC1 was cloned into pET28A for the 6 × His tag and into pCMVtag1 for the myc tag. Full length DISC1 (amino acids 1-854), mDISC1ΔNT (amino acids 347-854), CT (amino acids 633-854), MD (amino acids 347-633), mDISC1ΔCT (amino acids 1-633), NT (amino acids 1-347) were cloned into pRK5 for the HA tag.
