neurons than neurons treated at 10 and 25 mM concentrations for 48 h. On the other hand, mature neurons showed a moderate but significant viability loss at 50 and 75 mM EtOH treatments for 48 h post treatments compared to neural progenitors and immature neurons. Differences in cellular metabolic activities and viability between mature neuron cultures and neurospheres, progenitors, and immature neurons were also analyzed and compared at 6, 24, and 48 h post treatments for the 50 mM EtOH exposure. The results suggest that the difference in metabolic activity and cellular viability reduction induced by EtOH exposure was quite significant between mature neurons and neural progenitors as well as neurospheres (Fig. 2e). On the other hand, the reduction rate of viability loss in immature neurons was not significantly different than those for the mature neuron cultures exposed to 50 mM EtOH up to 48 h post treatments.
