cells lost their viability at 50 and 75 mm concentrations for 24 and 48 h post exposures. hNPCs also showed reduced metabolic rates and loss of cell viability with a significant decrease at 25, 50, and 75 mM concentrations at 6, 24 or 48 h (Fig. 2b). The effect was highly dramatic for the 24 and 48 h post exposures at 50 and 75 mM concentrations. Immature neuron cultures showed a significant cell viability loss at 6 and 24 h post exposures mainly at 50 and 75 mM EtOH concentrations and 25 mM and higher concentrations at 48 h post exposures (Fig. 2c). Interestingly, mature neuron cultures were resistant to the toxic effects of EtOH for the initial 6 h post treatments up to 75 mM concentrations. Mature neuron cultures showed low to moderate but significant viability loss at 10 to 75 mM concentrations at 24 h post exposures. Interestingly, viability loss was recovered with no significant difference of control neurons than neurons treated at 10 and 25 mM concentrations for 48 h. On the other hand, mature neurons showed a moderate but significant viability loss at 50 and 75 mM EtOH treatments for 48 h post treatments compared to
