Chunk #30 — Results — Organoids derived from schizophrenia iPSCs—dispersion of proliferating cells in the cortex and blockade of cortical neuronal development
Staining with a monoclonal Pan-Neu antibody, which reacts with key somatic, nuclear, dendritic, and axonal proteins of the pan-neuronal architecture, revealed differentiated Pan-Neu+ neurons concentrated in the CZ of the control iPSC organoids, forming a distinct cortical layer at 2 weeks (Fig. 2a) and more pronounced at 5 weeks (Fig. 2b). These mature neurons formed a dense network of long processes parallel, as well as perpendicular to the cortical surface. At 2 weeks, the schizophrenia iPSC organoids had noticeably fewer Pan-Neu neurons and Pan-Neu positive dendrites in the CZ (Fig. 2a, Supplementary Fig. 2b). Instead, the schizophrenia organoids displayed differentiated Pan-Neu+ neurons deep within the IZ and VZ regions. These mature subcortical neurons were found already at 2 weeks in the schizophrenia organoids, at the time when no such neurons were observed in the control organoids (Fig. 2a, Supplementary Fig. 2b). At 5 weeks, the basal cortical Pan-Neu positive neurons in schizophrenia organoids displayed dense short processes, different from the network of the long processes formed in the cortex of the control organoids (Fig. 2b). Overall, density of the Pan-Neu
