cultures, which do not preserve the genetic information relevant to disease inherent to patient-specific iPS cell-derived neurons. Reported compartmentalized systems have been shown to capture certain pathological hallmarks of neuropsychiatric disorders, but are often not conducive to parallelization for high-throughput screening (HTS). Furthermore, human neurons have been utilized in HTS platforms,14–18 but primarily with single cell populations, and not in contexts where distinct neuronal groups interact with one another in a defined connectivity.
