Recently, microfabrication has presented itself as a major tool for generating compartmentalized neural circuit models, many of which utilize a design and fabrication approach similar to that originally developed by the Jeon group2–6 in which connecting microchannels between compartments facilitate axonal extension while preventing cell body migration between adjacent chambers. While these compartmentalized devices were initially designed for modeling axonal injury, they demonstrated the utility of microfabrication to create similar devices for neuronal communication studies. Since then, a variety of devices have been developed based on this approach for the investigation of neuronal injury and circuit dynamics,7–11 some of which have recently translated measured circuit function into drug screening models producing disease-specific results.12,13 While the utility of compartmentalized devices in the study of neurocircuits has been demonstrated, the majority of reported studies have been limited to the use of primary cultures, which do not preserve the genetic information relevant to disease inherent to patient-specific iPS cell-derived neurons. Reported compartmentalized systems have been shown to capture certain pathological hallmarks of neuropsychiatric disorders, but are often not conducive to parallelization for high-throughput
