both the first and last exons of genes (P = 0.0008 and P = 0.004, respectively; permutation test) whereas hypomethylated RDme are significantly depleted inside the first exon (P = 0.0022; permutation test; Fig. 3b – red for hypermethylated RDme and blue for hypomethylated RDme). In addition, we observed an enrichment of hypomethylated RDme upstream of the TSS (P = 0.02; Fig. 3b – blue line). These data showing an enrichment of hyperacetylated RDac and hypermethylated RDme within exons and an enrichment of hypomethylated RDme in regulatory elements are consistent with previous data in cancer cells showing high exonic H3K9 acetylation [25], [26], [27] and DNA methylation [29] and low promoter DNA methylation associated with actively transcribed genes.
