Next, we performed an analysis of probe-level changes in epigenetic and transcriptional signaling as an alternative method to compare to previous studies in cancer. We compared probe-level differences in H3K9 acetylation, DNA methylation, and RNA transcription to (1) identify whether our data show a similar correspondence between higher levels of transcription observed in the High LG offspring and epigenetic changes we expect based on studies in cancer cells and (2) examine whether the observed patterns at the level of individual probes are indicative of our analyses of RDme and RDac. Figure 3c shows differences in H3K9 acetylation, DNA methylation, and RNA expression, with non-zero values indicating significant differences between High and Low LG offspring and line thickness denoting the standard error of the mean. In agreement with previous studies in cancer and the analyses of RDac/me above, H3K9 acetylation levels are significantly higher inside exons of the High LG offspring compared to the Low LG offspring, particularly the first and last exons ( Fig. 3c – left panel). DNA methylation differences between the groups are, on average, significantly higher within
