are significantly higher inside exons of the High LG offspring compared to the Low LG offspring, particularly the first and last exons ( Fig. 3c – left panel). DNA methylation differences between the groups are, on average, significantly higher within exons among High LG offspring compared to Low LG offspring ( Fig. 3c – middle panel). Expression differences inside exons indicate that High LG offspring show, on average, significantly higher RNA expression within annotated genes among High LG offspring compared to Low LG offspring ( Fig. 3c – right panel). These data confirm previously published observations in cancer cells showing an association of actively transcribed genes with hyperacetylation and high methylation within exons [25], [26], [27], [29]. Taken together, these analyses within the regulatory and transcriptional elements of the genes in the locus are consistent with an observed significantly higher overall transcriptional activity among High LG adult offspring.
