We previously reported that NR3C1 gene expression and H3K9 acetylation were increased and DNA methylation was decreased in the promoter of the exon 17 splice variant among High LG offspring compared to Low LG offspring [6], [7]. Using our comprehensive coverage of the entire NR3C1 locus we were able to identify additional novel regions of differential transcription, DNA methylation and histone acetylation in response to maternal care. We observed a number of RDme and RDac co-localized within intronic regions and upstream of the promoter region within the NR3C1 gene ( Fig. 4a ). The NR3C1 gene is known to contain at least 11 untranslated 5′ exon 1 splice variants that encode a common protein via a splice acceptor site on the exon 2. In this way, tissue-specific expression of NR3C1 is regulated by alternative splicing [30]. Our gene expression data agrees with previous studies showing that the expression of the GR exon 17 splice variant as well as that of exon 2 is increased in High LG offspring ([6], [7]; Fig. 4b ). Furthermore, we also detected increased transcription among
